Peroxisome and Histone Protein Distribution in Muntjac Fibroblasts

In a double immunofluorescence labeling experiment, a culture of Indian Muntjac fibroblasts was treated with a cocktail of mouse anti-histones (pan) and rabbit anti-PMP 70 (peroxisomal membrane protein) primary antibodies, followed by goat anti-mouse and anti-rabbit secondary antibodies conjugated to Alexa Fluor 568 and Alexa Fluor 488, respectively, to target the nuclear histone proteins and peroxisomes. The actin cytoskeleton was visualized with Alexa Fluor 633 conjugated to phalloidin.