Indian Muntjac Deer Skin Cells Triple Labeled for Mitochondria, Filamentous Actin, and DNA

A triple fluorophore combination of MitoTracker Red CMXRos, Alexa Fluor 488 conjugated to phalloidin, and DRAQ5 was used to label the adherent culture of Indian Muntjac cells presented in this section for mitochondria, the F-actin network, and nuclear DNA, respectively. The cells were first treated with the MitoTracker probe in growth medium for one hour, washed and fixed with paraformaldehyde (prepared in growth medium), permeabilized, and blocked with bovine serum albumen. The cells were subsequently labeled with the conjugated phalloidin and counterstained with the anthraquinone (DRAQ5) reagent.