Muntjac Skin Cells Labeled for Tubulin with Immunofluorescence

In order to visualize the microtubules present in a culture of Indian Muntjac fibroblasts, the cells were immunofluorescently labeled with anti-tubulin mouse monoclonal primary antibodies followed by goat anti-mouse Fab fragments conjugated to Alexa Fluor 488. In addition, the cells were labeled for mitochondria with MitoTracker Red CMXRos, a derivative of X-rosamine, and DNA in the nucleus with the far-red fluorescent probe DRAQ5.