Distribution of F-Actin and Mitochondria in Muntjac Cell Cultures

The proximity between the mitochondrial network and the filamentous actin cytoskeleton was visualized by treating a culture of Indian Muntjac deer skin fibroblasts with MitoTracker Red CMXRos and Alexa Fluor 488 conjugated to phalloidin. The specimen was also probed with DRAQ5, a DNA-interactive reagent that exhibits preferential intercalation at AT base pairs.