Targeting Giantin in Cancer Cells with Immunofluorescence

The HeLa cell culture shown in this confocal image was fixed, permeabilized, washed, and blocked with 10-percent normal goat serum in phosphate-buffered saline prior to immunofluorescent labeling with rabbit primary antibodies to giantin, a protein resident in the Golgi complex of mammalian cells. The culture was subsequently stained with a mixture of secondary antibodies conjugated to Alexa Fluor 647. In addition, the filamentous actin network was probed with Alexa Fluor 488 conjugated to phalloidin, and DNA was labeled with the nucleic acid stain SYTOX Orange.