HeLa Cervical Carcinoma Cells Labeled with Alexa Fluor Dyes and TO-PRO-3

Immunofluorescence with mouse anti-alpha-tubulin was employed to visualize details of the microtubule network in a log phase monolayer culture of HeLa adenocarcinoma cells. The secondary antibody (goat anti-mouse IgG) was conjugated to Alexa Fluor 488 and mixed with Alexa Fluor 546 conjugated to phalloidin to simultaneously image tubulin and the actin cytoskeleton. Nuclei were counterstained with TO-PRO-3, a carbocyanine monomer with long-wavelength red fluorescence.