Microtubules Immunofluorescently Visualized in Mongolian Gerbil Lung Fibroblasts

The gerbil lung cells presented in this confocal image were resident in a culture fixed with 3.7 percent paraformaldehyde, permeabilized with Triton X-100, blocked with 10 percent normal goat serum and treated with mouse anti-alpha-tubulin primary antibodies. Goat anti-mouse secondary antibodies conjugated to Alexa Fluor 568 (pseudocolored green) were mixed with the DNA stain DRAQ5 in order to simultaneously visualize microtubules and cell nuclei.