Visualizing the Actin Cytoskeleton in Gerbil Lung Cells with BODIPY FL Conjugated to a Phallotoxin

Filamentous actin was targeted in a culture of GeLu fibroblasts with BODIPY FL conjugated to phallacidin, a bicyclic peptide derived from a toxic mushroom. In addition, the cells were treated with the mitochondrion-selective stain MitoTracker Red CMXRos and a nuclear counterstain, TO-PRO-3.