The Microtubule Network in Gerbil Lung Cells

Immunofluorescence with mouse anti-alpha-tubulin was employed to visualize details of the microtubule network in the log phase monolayer culture of gerbil lung cells presented in this section. The secondary antibody (goat anti-mouse IgG) was conjugated to Alexa Fluor 555 (pseudocolored green). Nuclei were counterstained with TO-PRO-3, a carbocyanine monomer with long-wavelength red fluorescence.