Targeting Nuclear Pore Complexes in Fox Lung Fibroblast Cells with Immunofluorescence

Nuclei in a culture of FoLu cells were stained with Hoechst 33342 and immunofluorescence was utilized to visualize the numerous nuclear pore complexes located on each nucleus. The cells were fixed, permeabilized, blocked with 10-percent normal goat serum, and treated with mouse anti-NPCP (nuclear pore complex protein) primary antibodies followed by goat anti-mouse secondary antibodies (IgG) conjugated to Alexa Fluor 647 (pseudocolored yellow). The mitochondrial network was also targeted by treating the fibroblasts with MitoTracker Red CMXRos.