Intermediate Filaments in FoLu Fox Lung Fibroblasts

In order to label the intermediate filaments in the log phase adherent FoLu culture shown in this confocal image, the fixed and permeabilized cells were blocked and treated with mouse anti-vimentin (porcine eye lens) primary antibodies followed by goat anti-mouse secondary antibodies (IgG) conjugated to Alexa Fluor 488. Cell nuclei were counterstained with the red-absorbing dye TO-PRO-3.