Immunofluorescently Targeting the Golgi Complex in Feline Kidney Cell Cultures

The culture of feline kidney (CRFK) cells shown in this confocal image was fixed with paraformaldehyde, permeabilized, and treated with rabbit (anti-giantin) primary antibodies, followed by secondary antibodies conjugated to Alexa Fluor 568, targeting the Golgi apparatus. DNA in the cell nucleus was counterstained with TO-PRO-3 (pseudocolored cyan).