Targeting the Actin Cytoskeleton in Endothelial Cells with Alexa Fluor Probes Conjugated to Phallotoxins

A log phase culture of bovine pulmonary artery cells was labeled with Alexa Fluor 568 (pseudocolored blue) conjugated to phalloidin in order to target the actin cytoskeleton. The cells were also stained with MitoTracker Deep Red 633 and YO-PRO-1, which preferentially bind with intracellular mitochondria and DNA in the cell nucleus, respectively.