Visualizing Intermediate Filaments and the Golgi Complex in BPAE Cells with Immunofluorescence

In a double immunofluorescence experiment, an adherent monolayer culture of BPAE cells was fixed, permeabilized, blocked with 10-percent normal goat serum, and then treated with a cocktail of mouse anti-vimentin (intermediate filaments) and rabbit anti-GPP130 (Golgi complex) primary antibodies followed by goat anti-mouse and anti-rabbit secondary antibodies (IgG) conjugated to Alexa Fluor 568 (pseudocolored blue) and Alexa Fluor 488, respectively.