BPAE Cells Probed with Alexa Fluor 488, MitoTracker Red CMXRos, and TO-PRO-3

The culture of BPAE cells presented in this confocal image was labeled for mitochondria with MitoTracker Red CMXRos and for the cytoskeletal filamentous actin network with Alexa Fluor 488 conjugated to phalloidin, a cyclic peptide derived from the toxic death cap mushroom (Amanita phalloides). In addition, the cells were probed for nuclear DNA with TO-PRO-3 (pseudocolored cyan), a monomeric cyanine nucleic acid stain.