Imaging the Mitochondrial and F-Actin Networks in Monolayer Thoracic Aorta Cell Cultures

A monolayer culture of A-10 myoblasts was treated with MitoTracker Red CMXRos in medium containing 15 percent Cosmic calf serum, fixed with the same medium containing 3.7 percent paraformaldehyde, permeabilized with 0.2 percent Triton X-100, and then counterstained with TO-PRO-3 (pseudocolored blue), targeting DNA in the nuclei. In addition, the filamentous actin network was labeled with Alexa Fluor 488 conjugated to phalloidin.