Distribution of Histone Proteins and Peroxisomes in 3T3 Cultures

Nuclear histone proteins were targeted in the culture of 3T3 cells presented in this section with mouse anti-histone (pan) monoclonal antibodies, which were imaged with goat anti-mouse Fab fragments conjugated to Alexa Fluor 568 (labeling the nucleus). The specimen was simultaneously labeled for peroxisomes with Alexa Fluor 488 conjugated to goat secondary antibodies that target rabbit anti-PMP 70 (peroxisomal membrane protein 70).