Microtubule Network in Albino Swiss Mouse Fibroblast Cells

In order to visualize the microtubules present in a culture of 3T3 fibroblasts, the cells were immunofluorescently labeled with anti-tubulin mouse monoclonal primary antibodies followed by goat anti-mouse Fab fragments conjugated to Alexa Fluor 555. In addition, the cells were labeled for DNA in the nucleus with the red-absorbing dye TO-PRO-3.