Tubulin, Actin, and DNA Distribution in 3T3 Cells

A culture of 3T3 cells was immunofluorescently labeled with primary anti-tubulin mouse monoclonal antibodies followed by goat anti-mouse secondary antibodies conjugated to Alexa Fluor 488. In addition, the cells were simultaneously probed for DNA with the red-absorbing probe TO-PRO-3, and for the cytoskeletal filamentous actin network with Alexa Fluor 568 conjugated to phalloidin.